LEGEND MAX™ Total TGF-b1 ELISA Kit with Pre-coated Plates 5 Pre-coated Plates
Transforming growth factor beta 1 (TGF-β1) is a member of the transforming growth factor beta superfamily of cytokines. TGF-ß1 precursor contains 390 amino acids with an N-terminal signal peptide of 29 amino acids required for secretion from a cell, a 249 amino acids pro-region ( latency associated peptide or LAP), and a 112 amino acids C-terminal region that becomes the active TGF-β 1 upon activation.
Both LAP and TGF-ß1 exist as homodimers in circulation, but the disulfide linked homodimers of LAP and TGF-β1 remain non-covalently associated, forming the small latent TGFβ1 complex (SLC, 100 kD). The large latent TGF- β1 Complex (LLC, 235 – 260 kD) contains a third component, the latent TGF- β binding protein (LTBP), which is linked to LAP by a single disulfide bond. The LTBP does not confer latency, but for efficient secretion of the complex to extracellular sites. Free active TGF-β1 can be released (activated) by many factors including enzymes and low or high pH.
TGF-β1 is nearly 100% conserved across mammalian species. It has diverse biological functions in multiple cellular processes such as regulating proliferation and differentiation of various cell types. TGF-ß1 is also an important immunoregulatory cytokine, which is involved in the maintenance of self-tolerance, Th17 differentiation, and T cell homeostasis etc.
- Reactivity
- Human, Mouse, Rat, Non-human Primates, Bovine, Porcine, Ovine, Rabbit.
- Application
ELISA
- Application References
(PubMed link indicates BioLegend citation)
- Park GB, et al. 2014. Invest Opthalmol Vis Sci. 55:1770. PubMed
- Johannesson B, et al. 2014. Dis Model Mech. 7:977. PubMed
- Park G, et al. 2014. Invest Ophthalmol Vis Sci . 55:1770. PubMed
- Johannesson B, et al. 2014. Dis Model Mech. 7:977. PubMed
- O’Conor C, et al. 2016. Sci Rep. 6:29053. PubMed
- Chen C, et al. 2016. J Lipid Res. 57: 1435 – 1446. PubMed
- Choi J, et al. 2017. Sci Rep. 7:43349. PubMed
- Sensitivity
- 3.5 pg/mL
- Standard Range
- 7.8-500 pg/mL
- Materials Not Included
- Microplate reader able to measure absorbance at 450 nm
- Adjustable pipettes to measure volumes ranging from 1 µL to 1,000 µL
- Deionized water
- Wash bottle or automated microplate washer
- Log-Log graph paper or software for data analysis
- Tubes to prepare standard dilutions
- Timer
- Plate Shaker
- Polypropylene vials
- Kit Contents
- Anti-TGF-β1 Pre-coated 96-well Strip Microplate
- TGF-β1 Dectection Antibody
- TGF-β1 Standard
- Avidin-HRP D
- Assay Buffer C
- Wash Buffer (20X)
- Substrate Solution F
- Sample Diluent
- Acidification Solution
- Neutralization Solution
- Stop Solution
- Plate Sealers
- Cell Sources
- Many cell types, highly expressed on activated Tregs and platelets
- Gene ID
- 21803View all products for this Gene ID
- UniProt
- View information about TGF-beta1 on UniProt.org
Reviews
There are no reviews yet.