Rat TNF-a ELISA MAX™ Deluxe 20 Plates | Medical Supply Company

Rat TNF-a ELISA MAX™ Deluxe 5 Plates

SKU: 438204 Categories: Deluxe Set, ELISA, Rat, Sandwich Tag: Biolegend BV

Description
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Rat TNF-a ELISA MAX™ Deluxe 5 Plates

TNF-α (Tumor Necrosis Factor α), also termed Cachectin, Cytotoxic Factor (CF), Hemorrhagic Factor, Macrophage Cytotoxic Factor (MCF), is a potent multifunctional cytokine which can exert regulatory, inflammatory and cytotoxic effects on a wide range of normal lymphoid, non-lymphoid cells, and tumor cells. TNF-α is secreted by macrophages, monocytes, neutrophils, T-cells, NK-cells, and many transformed cell lines. It exists as a soluble 17.5 kD protein and a 26 kD membrane-associated protein. The soluble form protein derives from the membrane form by proteolytic processing. Dephosphorylation of the membrane form occurs by binding to soluble TNFRSF1A/TNFR1. TNF-α binding to surface receptors elicits a wide array of biological activities including: cytolysis and cytostasis of many tumor cell lines in vitro, hemorrhagic necrosis of tumors in vivo, increased fibroblast proliferation, and enhanced chemotaxis and phagocytosis in neutrophils. It has been shown that TNF-α plays a critical role in many physiological and pathological conditions including neurodegenerative diseases and cancer.

This kit is specifically designed for the accurate quantitation of Rat TNF-α from cell culture supernatant, serum, plasma, or other biological fluids. It is designed to meet the increasing use of rat models in biomedical studies.

Reactivity

Rat

Application

ELISA

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Application References (PubMed link indicates BioLegend citation)

Murugesan P, et al. 2016. J Infect Dis. 213: 532 – 540. PubMed
Hajighorbani M, et al. 2017. Inflammopharmacology. 10.1007/s10787-017-0335-2. PubMed
Schmidt K, et al. 2017. Microvasc Res. 10.1016/j.mvr.2017.08.002. PubMed

Sensitivity

2 pg/ml

Standard Range

15.6-1,000 pg/mL

Materials Not Included

A microplate reader capable of measuring absorbance at 450 nm
Adjustable pipettes to measure volumes ranging from 2 µL to 1 mL
Deionized (DI) water
PBS (Phosphate-Buffered Saline): 8.0 g NaCl, 1.16 g Na₂HPO₄, 0.2 g KH₂PO₄, 0.2 g KCl, add deionized water to 1 L; pH to 7.4, 0.2 µm filtered.
Wash Buffer (Phosphate-Buffered Saline (PBS) + 0.05% Tween-20, pH=7.4). BioLegend Cat. No. 421601 is recommended.
Wash bottle or automated microplate washer
Stop Solution (e.g. 2N H₂SO₄) Biolegend Cat. No. 423001 is recommended
Log-Log graph paper or software for data analysis
Tubes to prepare standard dilutions
Timer
Plate Sealer
Absorbent paper

Kit Contents

Rat TNF-α ELISA MAX™ Capture Antibody (200X)
Rat TNF-α ELISA MAX™ Detection Antibody (200X)
Rat TNF-α Standard
Avidin-HRP (1000X)
Substrate Solution A
Substrate Solution B
Coating Buffer A (5X)
Assay Diluent A (5X)
Matrix Diluent A (For serum and plasma samples only)
NUNC Maxisorp™ 96 MicroWell Plates

Cell Sources: Activated monocytes, neutrophils, macrophages, T cells, B cells, NK cells, LAK cells
Gene ID: 24835View all products for this Gene ID
UniProt: View information about TNF-alpha on UniProt.org