RiboShield™ RNase Inhibitor

Description

RNase Inhibition

Ribonucleases (RNases) are found in all cell types and organisms, and their ubiquitous nature can be highly problematic in the lab. Contamination with even the smallest amount of RNase in an RNA sample is enough to completely destroy the RNA. RiboShield™ RNase Inhibitor is a recombinant human placental protein that blocks the activity of a wide range of ribonucleases, including RNase A, B and C, to reliably protect your RNA from RNase digestion.

Superior RNA protection

RiboShield™ RNase Inhibitor is designed for RNA-sensitive applications including RNA isolation, RT-qPCR and RNA sequencing, to shield your RNA from degradation and provide higher yields and better performance as a result.

The inhibitor binds noncovalently to RNases at a 1:1 ratio and with very rapid kinetics of association, guaranteeing immediate protection of your RNA. RiboShield™ RNase Inhibitor has been tested in RT-qPCR and offers the greatest RNA protection when compared to leading competitor products (see figure 1).

Stability at higher temperatures

RiboShield™ RNase Inhibitor is able to perform over a wide range of reaction conditions and can sustain inhibition of RNase A at temperatures up to 65°C for at least 30 minutes (see figure 2). In addition, RiboShield™ does not contain cysteine residues that have been implicated in the oxidation sensitivity of the human placental version of the protein¹. This results in an RNase inhibitor molecule that is not only thermostable, but also more resistant to oxidative stress.

Flexible

The inhibitor can be used to block the activity of a wide range of ribonucleases, including eukaryotic RNases of the neutral type (RNases A, B and C). It does not inhibit RNases T1, T2, U1, U2, CL3, RNase I and H. The inhibitor does not hinder other enzymes such as reverse transcriptases, RNA polymerases or Taq DNA polymerase making it compatible with many enzymatic reactions involving RNA.

RiboShield™ is purified from a strain of Pichia pastoris expressing a modified human placental gene. The inhibitor is free from ribonucleases and phophatases and is inactivated by heating at 75°C for 15 minutes.

¹ Kim BM, Schultz LW, Raines RT. Variants of ribonuclease inhibitor that resist oxidation. Protein Science. 1999; 8(2):430-434. 

Features

• Superior protection leading to better performance of RNA-sensitive applications
• Particularly suited to incorporation into saliva-based tests for SARS-CoV-2 detection
• Inhibits eukaryotic RNases, including RNase A, B and C
• Compatible with reverse transcriptases, RNA polymerases and Taq DNA polymerase
• Stable up to 65°C for at least 30 minutes
• Ribonuclease and phosphatase free
• Ideal for long term storage of samples

Applications

• cDNA synthesis
• 1-step RT-PCR
• 1-step RT-qPCR
• RNA purification
• RNA sequencing
• In vitro transcription and translation
• Saliva-based diagnostic testing for SARS-CoV-2

Size

2,500 units