The Total RNA Extraction Kit (Tissue) is specially designed for purification of total RNA from a variety of animal tissues or cells. The provided micropestle (Mini Format) efficiently homogenizes tissue samples in a microcentrifuge tube. The method uses detergents and a chaotropic salt to lyse cell and inactivate RNase. RNA in the chaotropic salt solution is bound to the glass fiber matrix of the column. Following washing off of the contaminants the purified RNA is eluted by RNase-free water. The entire procedure can be completed in 20-40 minutes. ssRNA and dsRNA of > 200 bps to 1000′s of bps in length are efficiently purified.