FrameStar® 96 Well Semi-Skirted PCR Plate, ABI® Style, Low Binding

Name: FrameStar® 96 Well Semi-Skirted PCR Plate, ABI® Style, Low Binding | Medical Supply Company
Brand: 4titude
SKU: 4ti-LB0770/C

Standard profile, 0.2 ml polypropylene wells, polycarbonate frame, cut corner A12, working volume: <200 µl, total well capacity: 300 µl; designed for use on all major cyclers, including ABI® instruments with standard 96 well blocks; now also available as low binding plate with ultra-low DNA binding properties

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SKU: 4ti-LB0770/C Categories: Genomics Plastics, Plastics & Consumables Tag: 4titude

Description

Standard profile, 0.2 ml polypropylene wells, polycarbonate frame, cut corner A12, working volume: <200 µl, total well capacity: 300 µl; designed for use on all major cyclers, including ABI® instruments with standard 96 well blocks

Specifically designed to be directly compatible with all major thermal cyclers, this plate can be used directly in ABI® 96 well instruments without the need for any adaptors.

The rigid FrameStar® 2-component design eliminates warping and distortion during PCR, making it ideal for use with robotic systems. The semi-skirt allows for labelling or barcoding for sample tracking.

Perfect for ABI® thermal cyclers & sequencers

Recommended for low volume PCR

Rigid polycarbonate frame adds to the mechanical stability of the plate – as a result thermal expansion and sample evaporation is reduced significantly, leading to an improved consistency in PCR results

Ultra-smooth, uniform, thin-walled polypropylene tubes for optimum PCR and real-time (RT-qPCR) results

Improved seal integrity due to thermal stability of frame

Compatible with standard multichannel pipettes

Ideal for use with robotic systems

<0.2 ml (200 µl) working volume, 0.3 ml (300 µl) total well capacity

Raised and colour differentiated grid reference to aid well and sample identification

Available barcoded

6 frame colour options for the clear well variety – purple, blue, clear, green, red, and black

Also available as a black frame with white wells for optimum signal-to-noise ratio when using fluorescent based assays; and as a clear frame with frosted wells (see below)

NEW –FrameStar 96 Lid available

Now Available – Low Binding Plates with Ultra-Low DNA Binding Properties

Sensitive applications such as Next Generation Sequencing sample preparation applications demand the highest quality consumables. Low binding polypropylene tubes allow for multiple incubation and transfer steps without sample loss.

Polypropylene (PP) is the best plastic material for PCR tubes as it is chemically inert, resistant to solvents, and well suited for injection moulding – allowing for production of thin-walled tubes for optimum PCR results.

DNA has been shown to bind to PP tubes especially at high ionic strength, despite the very hydrophobic nature of this material. Different PP polymers are used for the production of PCR consumables and as they differ in their characteristics including surface charges, they consequently bind DNA in varying amounts.

DNA binding to PP surfaces has typically only been an issue for reaction tubes and storage vessels but not for PCR/qPCR tubes. DNA sticking to tube walls is either released during denaturation steps and/or remains accessible for amplification.

Nevertheless, due to a progressing volume miniaturisation and with new technologies such as Next Generation Sequencing (NGS), PCR/qPCR tubes are more and more also recommended for other applications which may require ultra-low DNA binding.

Plates of this design with ultra-low DNA binding properties are now available to order under the product code 4ti-LB0770/C.

Low-binding feature results from selected low-bind polymers, no coating is used to achieve the binding characteristics – No contamination or modification of the samples
Tested under a broad temperature range – Maximum DNA recovery after low temperature storage and high temperature incubation
No DNA loss during incubation steps and sample transfer in NGS sample prep and library construction – Ideally suited for sensitive applications with ultra-low DNA input

Now Available – Frosted Wells for use with ABI®/LifeTechnologies® qPCR Instruments

While clear wells are the best for sample visibility and optical appeal, white wells increase the signal-to-noise ratio by maximising reflect of light in fluorescent based assays used in real-time PCR and qPCR. This leads to a better sensitivity and reproducibility from a wide range of qPCR instruments from most manufacturers.

However, white well PCR plates may lead to software related problems with ABI®/LifeTechnologies® cyclers. For this reason the manufacturer recommends the use of plates with frosted wells as an alternative to white wells. Although the frosting does not provide the same sensitivity as white wells, readings are still considerably higher than those achieved with standard clear wells.

In response to this, we at 4titude® have developed a frosted well variety of the FrameStar® 96 Well Semi-Skirted Plate to offer ABI®/LifeTechnologies® instrument users the time and money-saving advantages of FrameStar®. And as the signal levels are directly comparable with ABI®/LifeTechnologies® plates, there’s no need for users to recalibrate their instruments.

Find these plates in the Order tab under product code 4ti-0772.

Frosted wells compared to clear wells

Now Available – Ethylene Oxide Treated Plates

Forensic applications demand the highest quality consumables. Avoiding external DNA contamination is of the utmost importance, and as a result high demands are placed upon manufacturers of products for use throughout the workflow.

At 4titude®, we have a stringently controlled cleanroom production facility certified, according to ISO 9001 and ISO 13485, for production of DNA and RNA free products. Following cleanroom production, 4titude®’s consumables for forensic use are packed into ethylene oxide permeable bags and then subjected to ethylene oxide sterilisation. This 2-step processing ensures degradation of amplifiable DNA.

Plates of this design for forensic use are now available to order under the product code 4ti-OX770C/SBC.

Barcoding

This plate is available with linear Code 128-format barcodes to aid identification and traceability for your samples. To order your plates with barcodes, simply add /SBC to the end of the ordering code for Standard Single Barcoding, /DBC for Standard Double Barcoding, /TBC for Standard Triple Barcoding, or /QBC for Standard Quadruple Barcoding (see Barcoding for more details).

Plate Width	124.26 ± 0.25 mm
Plate Depth	83.97 ± 0.25 mm
Plate Height	20.70 ± 0.25 mm
Well Depth	20.20 ± 0.10 mm
Well Diameter	5.46 ± 0.10 mm
Distance to centre of A1 from top edge	10.495 ± 0.25 mm
Distance to centre of A1 from left edge	12.63 ± 0.25 mm
Pitch (distance between A1 and A2)	9.00 mm