LEGEND MAX™ Human CCL13 (MCP-4) ELISA Kit with Pre-coated Plates 5 Pre-coated Plates
CCL13 is a member of the CC family of chemokines, also known as MCP-4. Its gene is located on human chromosome 17 within a large cluster of other CC chemokines. The precursor of CCL13 protein contains 98 amino acids. Differential signal peptide cleavage results in multiple isoforms of CCL13, including one major form (long chain, aa 17-98), and two minor forms (short chain, aa 24-98 and medium chain, aa 22-98). Human CCL13 shares amino acid sequence identity with human CCL2 (MCP-1, 61%), CCL7 (MCP-3, 58%), CCL8 (MCP-2, 56%), CCL11 (Eotaxin-1, 64%), CCL24 (Eotaxin-2, 48%) and CCL26 (Eotaxin-3, 45%). It also shares 69% amino acid identity with dog and equine CCL13, respectively. The mouse and rat counterparts of human CCL13 appear to be CCL2, which share an amino acid sequence identity of 56% and 52% to the human CCL13.
CCL13 is expressed by lymphocytes, monocytes/macrophages, basophils and eosinophils, but not neutrophils. Its expression can be upregulated by the inflammatory cytokines interleukin-1 and TNF-α. IFN-γ also induces CCL13 in bronchial epithelial cell lines, endothelial cells, and chondrocytes, and a synergistic effect is obtained with IFN-γ, and TNF-α or IL-1β, in chondrocytes. CCL13 is processed and inactivated by MMP-12.
CCL13 acts as a chemoattractant for eosinophils, basophils, monocytes, macrophages, immature dendritic cells, and T cells through binding to the G-protein coupled receptors (CCR2, CCR3, and CCR5) expressed on these cells. A number of studies have shown that CCL13 is involved in many inflammatory diseases, in which it functions as a pivotal molecule involved in the selective recruitment of cell lineages to the inflamed tissues and their subsequent activation. High levels of CCL13 have been detected in the synovial fluid of patients with RA, in serum of asthmatic patients, and in plasma of patients with symptomatic carotid atherosclerosis.
The LEGEND MAX™ Human CCL13 (MCP-4) ELISA kit is a Sandwich Enzyme-Linked Immunosorbent Assay (ELISA) with a 96-well strip plate that is pre-coated with a mouse monoclonal anti-human CCL13 (MCP-4) antibody. The detection antibody is a biotinylated mouse monoclonal anti-human CCL13 (MCP-4) antibody. This kit is specifically designed for the accurate quantitation of human CCL13 (MCP-4) from cell culture supernatant, serum and plasma samples. This kit is analytically validated with ready-to-use reagents.
- Reactivity
- Human
- Application
ELISA
- Sensitivity
- 2.4 ± 0.4 pg/mL
- Standard Range
- 15.6 – 1,000 pg/mL
- Materials Not Included
- Microplate reader able to measure absorbance at 450 nm
- Adjustable pipettes to measure volumes ranging from 1 µl to 1,000 µl
- Deionized water
- Wash bottle or automated microplate washer
- Log-Log graph paper or software for data analysis
- Tubes to prepare standard dilutions
- Timer
- Plate Shaker
- Polypropylene vials
- Kit Contents
- Anti-Human CCL13 Pre-coated 96 well Strip Microplate
- Human CCL13 Detection Antibody
- Human CCL13 Standard
- Avidin-HRP
- Assay Buffer B
- Wash Buffer (20X)
- Substrate Solution F
- Stop Solution
- Plate Sealers
- Gene ID
- 6357View all products for this Gene ID
- UniProt
- View information about CCL13 on UniProt.org
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