LEGEND MAX™ Human IL-1B ELISA Kit with Pre-coated Plates 5 Pre-coated Plates

SKU: 437008 Categories: ELISA, Human, Pre-Coated, Sandwich Tag: Biolegend BV

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LEGEND MAX™ Human IL-1B ELISA Kit with Pre-coated Plates 5 Pre-coated Plates

Human IL-1β (Interleukin-1β, Catabolin, Hematopoietin-1 (H1), IFN-β-inducing factor, Interleukin-β, Osteoclast activating factor (OAF)). IL-1 refers to two proteins, IL-1α and IL-1β which are the products of distinct genes, but which are recognized by the same cell surface receptors. IL-1β is a potent immuno-modulator which mediates a wide range of immune and inflammatory responses including the activation of B and T cells. IL-1β is expressed in several cell types, including monocytes, tissue macrophages, Langerhan cells, dendritic cells, T lymphocytes, B lymphocytes, and natural killer cells. IL-1 β is upregulated by TNF-α, IFN-α, IFN-β, IFN-γ, bacterial endotoxins, viruses, mitogens, and antigens; while IL-1β is downregulated by IL-6, lipoproteins, lipids, and α2-macroglobulin. IL-1 β plays a role in many physiological and pathological conditions including rheumatoid arthritis and cancer.

BioLegend’s LEGEND MAX™ Human IL-1β ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay (ELISA), in which, a Human IL-1β specific monoclonal antibody is pre-coated on a 96-well strip-well plate.

BioLegend’s LEGEND MAX™ Human IL-1β ELISA Kit is specifically designed for the accurate quantitation of Human IL-1β from cell culture supernatant, serum, plasma or other bodily fluids. It is ready-to-use, accurate, and sensitive.

Reactivity

Human

Application

ELISA

Application Notes

BioLegend’s mouse and human IL-1β ELISA kits use antibodies raised against the mature form of IL-1β. However, because pro-IL-1β contains the mature form, these antibodies likely recognize both the pro- and the mature forms of the protein. The human IL-1β ELISA kits have been shown to equally recognize both pro- and mature forms of IL-1β.

Application References (PubMed link indicates BioLegend citation)

Jayaprakash K, et al. 2017. Acta Pathologica, Microbiologica et Immunologica Scandinavica. 10.1111/apm.12701. PubMed
Liu Y, et al. 2017. Int Immunopharmacol. 10.1016/j.intimp.2017.04.029. PubMed
Wang X, et al. 2017. Eur J Pharmacol. 10.1016/j.ejphar.2017.05.004. PubMed
Velagapudi R, et al. 2017. Int Immunopharmacol. 10.1016/j.intimp.2017.04.018. PubMed
Lin C, et al. 2017. Biochem Pharmacol. 10.1016/j.bcp.2017.06.118. PubMed
Zhang Z, et al. 2017. Int Immunopharmacol. 10.1016/j.intimp.2017.05.033. PubMed
Chen D, et al. 2017. Microb Pathog. 10.1016/j.micpath.2017.06.037. PubMed
Yu B, et al. 2017. Microb Pathog. 10.1016/j.micpath.2017.07.023. PubMed
Dong L, et al. 2017. Eur J Pharmacol. 10.1016/j.ejphar.2017.07.029. PubMed
Jantaruk P, et al. 2017. PLoS One.. 10.1371/journal.pone.0183852. PubMed

Sensitivity

0.5 pg/mL

Standard Range

2.0-125 pg/mL

Materials Not Included

Microplate reader able to measure absorbance at 450 nm
Adjustable pipettes to measure volumes ranging from 1 µL to 1,000 µL
Deionized water
Wash bottle or automated microplate washer
Log-Log graph paper or software for data analysis
Tubes to prepare standard dilutions
Timer
Plate Shaker
Polypropylene vials

Kit Contents

Anti-Human IL-1β Pre-coated 96-well Strip Microplate
Human IL-1β Dectection Antibody
Human IL-1β Standard
Avidin-HRP D
Assay Buffer D
Matrix E (for serum and plasma samples only)
Wash Buffer (20X)
Substrate Solution F
Stop Solution
Plate Sealers

Cell Sources: Monocytes, tissue macrophages, Langerhans cells, dendritic cells, T and B cells, natural killer (NK) cells, large granular lymphocytes (LGL), vascular endothelium, smooth muscle, fibroblasts, thymic epithelia, astrocytes, microglia, glioma, keratinocytes,
Gene ID: 3553View all products for this Gene ID
UniProt: View information about IL-1beta on UniProt.org