LEGEND MAX™ Human Total MMP-3 ELISA Kit with Pre-coated Plates 1 Pre-coated Plate

Matrix Metalloproteinase 3 (MMP-3), also known as Matrix Metallopeptidase 3, Stromelysin 1, Progelatinase, Proteoglycanase, or Transin-1, is a member of a broad family of MMPs, which are zinc-dependent enzymes that break down extracellular matrix proteins as part of their primary functions. MMP-3 is secreted in its 54 kD inactive pro-enzyme form from many cell types including fibroblasts, chondrocytes, endothelial cells, macrophages, vascular smooth muscle cells, osteoblasts, and keratinocytes. A cysteine residue in the pro-domain coordinates with the zinc in the catalytic domain to maintain latency. MMP-3 is activated by proteolytic proteins and compounds including MMP-7, 4-aminophenylmercuric acetate (APMA), and trypsin. Alpha macroglobulins and tissue inhibitor of metalloproteinase 1 (TIMP-1) are the primary natural inhibitors of MMP-3 that can regulate its activation and hinder its function. Activated MMP-3 degrades several forms of collagen, proteoglycan, fibronectin, laminin, and elastin.

Human MMP-3 shares significant amino acid sequence identity with MMP-3 from other species, including 85-99%, 83%, 81%, 77%, and 75% from non-human primates, bovine, equine, mouse, and rat, respectively.

MMP-3 is involved in tissue remodeling processes such as embryonic development, reproduction, morphogenesis, and angiogenesis. MMP-3 is a potential biomarker for many disease conditions, as it plays important roles in tumor growth, invasion, and metastasis. Additionally, MMP-3 activates several other MMP proteins by cleavage of the proenzyme domain.

The LEGEND MAX™ Human Total MMP-3 Sandwich Enzyme-Linked Immunosorbent Assay (ELISA) kit includes a 96-well strip plate that is pre-coated with a goat polyclonal anti-human MMP-3 antibody. The Detection Antibody is a biotinylated goat polyclonal anti-human MMP-3 antibody. This kit is specifically designed to accurately measure MMP-3, including Pro MMP-3, Mature MMP-3, and Mature MMP-3/TIMP-1 complex. This kit has been analytically validated to assess cell culture supernatant, serum, and heparin plasma samples with ready-to-use reagents.