LEGEND MAX™ Mouse TNF-a ELISA Kit with Pre-coated Plates 1 Pre-coated Plate
TNF-α is secreted by macrophages, monocytes, neutrophils, T-cells (principally CD4+), and NK-cells. Many transformed cell lines also secrete TNF-α. TNF-α forms multimeric complexes; stable trimers are most common in solution. A 26 kD membrane form of TNF-α has also been described. TNF-α binding to surface receptors elicits a wide array of biologic activities including: cytolysis and cytostasis of many tumor cell lines in vitro, hemorraghic necrosis of tumors in vivo, increased fibroblast proliferation, and enhanced chemotaxis and phagocytosis in neutrophils.
- Reactivity
- Mouse
- Application
ELISA
- Application References
(PubMed link indicates BioLegend citation)
- Kueanjinda P, Palaga S 2015. Sci Rep. 5: 12784. PubMed
- Lei G, et al. 2017. Antimicrob Agents Chemother. 10.1128/AAC.01226-17. PubMed
- Sensitivity
- 1.5 pg/ml
- Standard Range
- 15.6-1,000 pg/mL
- Materials Not Included
- Microplate reader able to measure absorbance at 450 nm
- Adjustable pipettes to measure volumes ranging from 1 µL to 1,000 µL
- Deionized water
- Wash bottle or automated microplate washer
- Log-Log graph paper or software for data analysis
- Tubes to prepare standard dilutions
- Timer
- Plate Shaker
- Polypropylene vials
- Kit Contents
- Anti-Mouse TNF-α Pre-coated 96-well Strip Microplate
- Mouse TNF-α Dectection Antibody
- Mouse TNF-α Standard
- Matrix C (for serum and plasma samples only)
- Avidin-HRP B
- Assay Buffer A
- Wash Buffer (20X)
- Substrate Solution D
- Stop Solution
- Plate Sealers
- Cell Sources
- Activated monocytes, neutrophils, macrophages, T cells, B cells, NK cells, LAK cells
- Gene ID
- 21926View all products for this Gene ID
- UniProt
- View information about TNF-alpha on UniProt.org
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