Mouse IL-12/IL-23 (p40) ELISA MAX™ Deluxe 20 plates | Medical Supply Company

Mouse IL-12/IL-23 (p40) ELISA MAX™ Standard 5 plates

SKU: 431601 Categories: ELISA, Mouse, Sandwich, Standard Set Tag: Biolegend BV

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Mouse IL-12/IL-23 (p40) ELISA MAX™ Standard 5 plates

Cytokine detection

IL-12, IL-23 and IL-35 share common subunits, utilizing combinations of p40, p19 and p35 proteins. Caution must be used when selecting antibodies and assays when specific identification, measurement, as well as activation state discrimination, is required.

1. Active IL-12 consists of two subunits: p40 + p35.
2. p40 can also exist as a monomer (IL-12 p40) or a homodimer (IL-12 p80).
3. Besides contributing to IL-12, p40 is also found in IL-23, a heterodimer of p40 and p19.
4. Similarly, p35 not only contributes to IL-12, but is also found in the heterodimer IL-35 (p35 and EBI3).

Note that assays using antibodies specific for the p40 subunit will be unable to discriminate between the active IL-12, monomeric p40, dimeric p40, and IL-23; likewise, assays using p35 detection alone will pick up both IL-12 (heterodimer) and IL-35.

BioLegend ELISA assays

ELISA Development Systems Engineered for Sensitive, Precise and Reliable Serum and Plasma Cytokine Quantitation

BioLegend’s ELISA Max™ Sets provide all the time-savings, convenience and performance of a high-end, commercial ELISA kit for a fraction of the price! BioLegend’s ELISA Max™ Sets, in two appealing formats, address the need for sensitivity, accuracy, dependability, and versatility of an ELISA development system, while providing maximum cost effectiveness, value, and ease of use.

Both the ELISA Max™ Set Standard and ELISA Max™ Set Deluxe are specifically engineered for accurate recovery and measurement of analytes in complex fluids, such as serum and plasma (as well as activated cell cultures) without any interference by confounding serum factors, such as heterophilic antibody or rheumatoid factor.

Reactivity

Mouse

Application

ELISA

As a part of BioLegend's efforts to go green, each new lot of the ELISA MAX™ sets will include a summarized version of its manual. Click here for more information.

Application References (PubMed link indicates BioLegend citation)

Combe CL, et al. 2005. Infect. Immun. 73:4913.
Gigley DJ, et al. 2006. P. Natl. Acad. Sci. USA 103:6635.
Stolk M, et al. 2006. J. Leukoc. Biol. 80:651.
O'Connell PJ, et al. 2006. Blood 107:1010.
Guan H, et al. 2007. J. Immunol. 179:590.
Moretto MM, et al. 2007. J. Immunol. 179:2485.
Espinosa-Cueto P, et al. 2017. PLoS One.. 10.1371/journal.pone.0182126. PubMed

Standard Range

7.8-500 pg/mL

Materials Not Included

Microwell plates: 96-well Nunc MaxiSorp™ is recommended.
A microplate reader capable of measuring absorbance at 450 nm
Adjustable pipettes to measure volumes ranging from 2 µL to 1 mL
Deionized (DI) water
Coating Buffer: 8.4 g NaHCO₃, 3.56 g Na₂CO₃, add DI H₂O to 1.0 L, pH to 9.5 (BioLegend Cat. No. 421701 is recommended.)
Assay Diluent: 10% Fetal Bovine Serum or 1% BSA in Phosphate-Buffered Saline (PBS) (BioLegend Cat. No. 421203 is recommended.)
PBS: 8.0 g NaCl, 1.16 g Na₂HPO₄, 0.2 g KH₂PO₄, 0.2 g KCl, add DI water to 1.0 L, pH to 7.4
Wash Buffer: Phosphate-Buffered Saline (PBS) + 0.05% Tween-20 (BioLegend Cat. No. 421601 is recommended.)
Wash bottle or automated microplate washer
TMB Substrate Solution – BioLegend Cat. No. 421101 is recommended.
Stop Solution (2 N H₂SO₄)
Log-Log graph paper or software for data analysis
Tubes to prepare standard dilutions
Timer
Absorbent paper