Mouse IL-10 ELISA MAX™ Standard 5 Plates
Mouse IL-10 was originally described as Cytokine Synthesis Inhibitory Factor (CSIF) due to its ability to inhibit cytokine production by TH1 clones. IL-10 is expressed in activated CD8+ and CD4+ T cells, activated monocytes, mast cells, and Ly-1 B cells. IL-10 shares over 80% sequence homology with the Epstein-Barr virus protein BCRFI. The functions of IL-10 include inhibition of macrophage-mediated cytokine synthesis, suppression of the delayed-type hypersensitivity response, and stimulation of the TH2 cell response, which results in elevated antibody production.
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Both the ELISA Max™ Set Standard and ELISA Max™ Set Deluxe are specifically engineered for accurate recovery and measurement of analytes in complex fluids, such as serum and plasma (as well as activated cell cultures) without any interference by confounding serum factors, such as heterophilic antibody or rheumatoid factor.
- Reactivity
- Mouse
- Application
ELISA
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- Application References
(PubMed link indicates BioLegend citation)
- Inoue S, et al. 2006. Cancer Research 66:7741.
- Lau AH, et al. 2006. J. Leukoc. Biol. 79:941.
- Kubo T, et al. 2009. J. Exp Med. 206:1971. PubMed
- Asai A, et al. 2012. Immunobiology. 217:711. PubMed
- Baca Jones C, et al. 2014. PLoS One. 9:90855. PubMed
- Asai A, et al. 2012. Immunobiology. 217:711. PubMed
- Jones C, et al. 2014. PLoS One. 9:90855. PubMed
- Murayama M, et al. 2015. Nat Commun. 6: 8483. PubMed
- Fenoy I, et al. 2015. Exp Parasitol. 154: 47-50. PubMed
- Fenoy IM, et al. 2015. Immunobiology. 22: 641-648. PubMed
- Egli N, et al. 2015. PLoS One. 10: 0142741. PubMed
- Singh N, et al. 2016. J Immunol. 196: 1108 – 1122. PubMed
- Croasdell A, et al. 2016. J Immunol. 196: 2742 – 2752. PubMed
- Andrade-Sousa A, et al. 2016. PLoS One. 11: 0163420. PubMed
- Th?nert R, et al. 2017. Nat Commun. 8:14268. PubMed
- Espinosa-Cueto P, et al. 2017. PLoS One.. 10.1371/journal.pone.0182126. PubMed
- Hu Y, et al. 2017. Biochim Biophys Acta. 10.1016/j.bbadis.2017.06.001. PubMed
- Standard Range
- 31.3-2,000 pg/mL
- Materials Not Included
- Microwell plates: 96-well Nunc MaxiSorp™ is recommended.
- A microplate reader capable of measuring absorbance at 450 nm
- Adjustable pipettes to measure volumes ranging from 2 µL to 1 mL
- Deionized (DI) water
- Coating Buffer: Phosphate-Buffered Saline (PBS), pH 7.4
- Assay Diluent: 10% Fetal Bovine Serum or 1% BSA in Phosphate-Buffered Saline (PBS) (BioLegend Cat. No. 421203 is recommended.)
- PBS: 8.0 g NaCl, 1.16 g Na2HPO4, 0.2 g KH2PO4, 0.2 g KCl, add DI water to 1.0 L, pH to 7.4
- Wash Buffer: Phosphate-Buffered Saline (PBS) + 0.05% Tween-20 (BioLegend Cat. No. 421601 is recommended.)
- Wash bottle or automated microplate washer
- TMB Substrate Solution – BioLegend Cat. No. 421101 is recommended.
- Stop Solution (2 N H2SO4)
- Log-Log graph paper or software for data analysis
- Tubes to prepare standard dilutions
- Timer
- Absorbent paper
- Kit Contents
- Mouse IL-10 ELISA MAX™ Capture Antibody (200X)
- Mouse IL-10 ELISA MAX™ Detection Antibody (200X)
- Mouse IL-10 Standard
- Avidin-HRP (1000X)
- Cell Sources
- Activated CD8+ T cells, Th0, Th2 subset of CD4+ T cells, Ly-1+ B cells, monocytes, macrophages, keratinocytes
- Gene ID
- 16153View all products for this Gene ID
- UniProt
- View information about IL-10 on UniProt.org
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