LEGEND MAX™ Mouse IL-10 ELISA Kit with Pre-coated Plates 5 Pre-coated Plates
Mouse IL-10 was originally described as Cytokine Synthesis Inhibitory Factor (CSIF) due to its ability to inhibit cytokine production by TH1 clones. IL-10 is expressed in activated CD8+ and CD4+ T cells, activated monocytes, mast cells, and Ly-1 B cells. IL-10 shares over 80% sequence homology with the Epstein-Barr virus protein BCRFI. The functions of IL-10 include inhibition of macrophage-mediated cytokine synthesis, suppression of the delayed-type hypersensitivity response, and stimulation of the TH2 cell response, which results in elevated antibody production.
The BioLegend LEGEND MAX™ Mouse IL-10 ELISA Kit is a Sandwich Enzyme-Linked Immunosorbent Assay (ELISA) with a 96-well strip plate that is pre-coated with a capture antibody. This kit is specifically designed for the accurate quantitation of mouse IL-10 from cell culture supernatant, serum, plasma, and other biological fluids. It is analytically validated with ready-to-use reagents.
- Reactivity
- Mouse
- Application
ELISA
- Application References
(PubMed link indicates BioLegend citation)
- Johannesson B, et al. 2014. Dis Model Mech. 7:977. PubMed
- McKelvey R, et al. 2015. J Neurosci. 35:457. PubMed
- Johannesson B, et al. 2014. Dis Model Mech. 7:977. PubMed
- McKelvey R, et al. 2015. J Neurosci. 35:457. PubMed
- Kueanjinda P, Palaga S 2015. Sci Rep. 5: 12784. PubMed
- Sensitivity
- 2.7 pg/mL
- Standard Range
- 15.6-1,000 pg/mL
- Materials Not Included
- Microplate reader able to measure absorbance at 450 nm
- Adjustable pipettes to measure volumes ranging from 1 µL to 1,000 µL
- Deionized water
- Wash bottle or automated microplate washer
- Log-Log graph paper or software for data analysis
- Tubes to prepare standard dilutions
- Timer
- Plate Shaker
- Polypropylene vials
- Kit Contents
- Anti-Mouse IL-10 Pre-coated 96-well Strip Microplate
- Mouse IL-10 Dectection Antibody
- Mouse IL-10 Standard
- Matrix C (for serum and plasma samples only)
- Avidin-HRP D
- Assay Buffer A
- Wash Buffer (20X)
- Substrate Solution F
- Stop Solution
- Plate Sealers
- Cell Sources
- Activated CD8+ T cells, Th0, Th2 subset of CD4+ T cells, Ly-1+ B cells, monocytes, macrophages, keratinocytes
- Gene ID
- 16153View all products for this Gene ID
- UniProt
- View information about IL-10 on UniProt.org
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