Mouse IL-12/IL-23 (p40) ELISA MAX™ Deluxe 5 plates
Cytokine detection
IL-12, IL-23 and IL-35 share common subunits, utilizing combinations of p40, p19 and p35 proteins. Caution must be used when selecting antibodies and assays when specific identification, measurement, as well as activation state discrimination, is required.
1. Active IL-12 consists of two subunits: p40 + p35.
2. p40 can also exist as a monomer (IL-12 p40) or a homodimer (IL-12 p80).
3. Besides contributing to IL-12, p40 is also found in IL-23, a heterodimer of p40 and p19.
4. Similarly, p35 not only contributes to IL-12, but is also found in the heterodimer IL-35 (p35 and EBI3).
Note that assays using antibodies specific for the p40 subunit will be unable to discriminate between the active IL-12, monomeric p40, dimeric p40, and IL-23; likewise, assays using p35 detection alone will pick up both IL-12 (heterodimer) and IL-35.
BioLegend ELISA assays
ELISA Development Systems Engineered for Sensitive, Precise and Reliable Serum and Plasma Cytokine Quantitation
BioLegend’s ELISA Max™ Sets provide all the time-savings, convenience and performance of a high-end, commercial ELISA kit for a fraction of the price!
BioLegend’s ELISA Max™ Sets, in two appealing formats, address the need for sensitivity, accuracy, dependability, and versatility of an ELISA development system, while providing maximum cost effectiveness, value, and ease of use.
Both the ELISA Max™ Set Standard and ELISA Max™ Set Deluxe are specifically engineered for accurate recovery and measurement of analytes in complex fluids, such as serum and plasma (as well as activated cell cultures) without any interference by confounding serum factors, such as heterophilic antibody or rheumatoid factor.
- Reactivity
- Mouse
- Application
ELISA
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- Application References
(PubMed link indicates BioLegend citation)
- Combe CL, et al. 2005. Infect. Immun. 73:4913.
- Gigley DJ, et al. 2006. P. Natl. Acad. Sci. USA 103:6635.
- Stolk M, et al. 2006. J. Leukoc. Biol. 80:651.
- O'Connell PJ, et al. 2006. Blood 107:1010.
- Guan H, et al. 2007. J. Immunol. 179:590.
- Moretto MM, et al. 2007. J. Immunol. 179:2485.
- Iqbal J, et al.2007. Am J Physiol Renal Physiol. 292:F1283. PubMed
- Zaidi J 2006. Am J Physiol Renal Physiol. 292:1283. PubMed
- Moretto M, et al. 2010. Infect Immun. 78:2505. PubMed
- Sensitivity
- 4 pg/mL
- Standard Range
- 7.8-500 pg/mL
- Materials Not Included
- A microplate reader capable of measuring absorbance at 450 nm
- Adjustable pipettes to measure volumes ranging from 2 µL to 1 mL
- Deionized (DI) water
- PBS (Phosphate-Buffered Saline): 8.0 g NaCl, 1.16 g Na2HPO4, 0.2 g KH2PO4, 0.2 g KCl, add deionized water to 1 L; pH to 7.4, 0.2 µm filtered.
- Wash Buffer (Phosphate-Buffered Saline (PBS) + 0.05% Tween-20, pH=7.4). BioLegend Cat. No. 421601 is recommended.
- Wash bottle or automated microplate washer
- Stop Solution (e.g. 2N H2SO4) Biolegend Cat. No. 423001 is recommended
- Log-Log graph paper or software for data analysis
- Tubes to prepare standard dilutions
- Timer
- Plate Sealer
- Absorbent paper
- Kit Contents
- Mouse IL-12/IL-23 (p40) ELISA MAX™ Capture Antibody (200X)
- Mouse IL-12/IL-23 (p40) ELISA MAX™ Detection Antibody (200X)
- Mouse IL-12/IL-23 (p40) Standard
- Avidin-HRP (1000X)
- Substrate Solution A
- Substrate Solution B
- Coating Buffer A (5X)
- Assay Diluent A (5X)
- NUNC Maxisorp™ 96 MicroWell Plates
- Cell Sources
- Dendritic cells, monocytes/macrophages, B cells, T cells
- Gene ID
- 16160View all products for this Gene ID
- UniProt
- View information about IL-12 p40 on UniProt.org
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