Mouse IL-10 ELISA MAX™ Deluxe 20 Plates | Medical Supply Company

Mouse IL-10 ELISA MAX™ Deluxe 5 Plates

SKU: 431414 Categories: Deluxe Set, ELISA, Mouse, Sandwich Tag: Biolegend BV

Description
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Mouse IL-10 ELISA MAX™ Deluxe 5 Plates

Mouse IL-10 was originally described as Cytokine Synthesis Inhibitory Factor (CSIF) due to its ability to inhibit cytokine production by TH1 clones. IL-10 is expressed in activated CD8⁺ and CD4⁺ T cells, activated monocytes, mast cells, and Ly-1 B cells. IL-10 shares over 80% sequence homology with the Epstein-Barr virus protein BCRFI. The functions of IL-10 include inhibition of macrophage-mediated cytokine synthesis, suppression of the delayed-type hypersensitivity response, and stimulation of the TH2 cell response, which results in elevated antibody production.

ELISA Development Systems Engineered for Sensitive, Precise and Reliable Serum and Plasma Cytokine Quantitation.

BioLegend’s ELISA Max™ Sets provide all the time-savings, convenience and performance of a high-end, commercial ELISA kit for a fraction of the price! BioLegend’s ELISA Max™ Sets, in two appealing formats, address the need for sensitivity, accuracy, dependability, and versatility of an ELISA development system, while providing maximum cost effectiveness, value, and ease of use.

Both the ELISA Max™ Set Standard and ELISA Max™ Set Deluxe are specifically engineered for accurate recovery and measurement of analytes in complex fluids, such as serum and plasma (as well as activated cell cultures) without any interference by confounding serum factors, such as heterophilic antibody or rheumatoid factor.

Reactivity

Mouse

Application

ELISA

As a part of BioLegend's efforts to go green, each new lot of the ELISA MAX™ sets will include a summarized version of its manual. Click here for more information.

Application References (PubMed link indicates BioLegend citation)

Inoue S, et al. 2006. Cancer Research 66:7741.
Lau AH, et al. 2006. J. Leukoc. Biol. 79:941.
Xiong A, et al. 2012. PLoS One. 7:e46230. PubMed.
Xu P, et al. 2013. J. Immunol. 190:5247. PubMed.
Xiong A, et al. 2012. PLoS One. 7:e46230. PubMed
Xu P, et al. 2013. J Immunol. 190:5247. PubMed
Naji A, et al. 2016. Sci Rep. 6: 26162. PubMed
Enomoto D, et al. 2017. PLoS One.. 10.1371/journal.pone.0183584. PubMed

Sensitivity

16 pg/mL

Standard Range

31.3-2,000 pg/mL

Materials Not Included

A microplate reader capable of measuring absorbance at 450 nm
Adjustable pipettes to measure volumes ranging from 2 µL to 1 mL
Deionized (DI) water
PBS (Phosphate-Buffered Saline): 8.0 g NaCl, 1.16 g Na₂HPO₄, 0.2 g KH₂PO₄, 0.2 g KCl, add deionized water to 1 L; pH to 7.4, 0.2 µm filtered.
Wash Buffer (Phosphate-Buffered Saline (PBS) + 0.05% Tween-20, pH=7.4). BioLegend Cat. No. 421601 is recommended.
Wash bottle or automated microplate washer
Stop Solution (e.g. 2N H₂SO₄) Biolegend Cat. No. 423001 is recommended
Log-Log graph paper or software for data analysis
Tubes to prepare standard dilutions
Timer
Plate Sealer
Absorbent paper

Kit Contents

Mouse IL-10 ELISA MAX™ Capture Antibody (200X)
Mouse IL-10 ELISA MAX™ Detection Antibody (200X)
Mouse IL-10 Standard
Avidin-HRP (1000X)
Substrate Solution A
Substrate Solution B
Coating Buffer B (5X)
Assay Diluent A (5X)
NUNC Maxisorp™ 96 MicroWell Plates

Cell Sources: Activated CD8⁺ T cells, Th0, Th2 subset of CD4⁺ T cells, Ly-1⁺ B cells, monocytes, macrophages, keratinocytes
Gene ID: 16153View all products for this Gene ID
UniProt: View information about IL-10 on UniProt.org