Mouse IL-4 ELISA MAX™ Standard 5 Plates

Mouse IL-4 ELISA MAX™ Standard 5 Plates

Reactivity

Mouse

Application

ELISA

 

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Application References

(PubMed link indicates BioLegend citation)

1. Tallima H, et. al. 2009. Cytokine 48:239. PubMed
2. El Ridi R, et al. 2010. Microbes Infect. 12:700. Ridi R, et al. 2010. Microbes Infect. 12:700. PubMed
3. Eyring K, et al. 2015. PLoS One. 10: 0144087. PubMed

Standard Range

2-125.0 pg/mL

Materials Not Included

• Microwell plates: 96-well Nunc MaxiSorp™ is recommended.
• A microplate reader capable of measuring absorbance at 450 nm
• Adjustable pipettes to measure volumes ranging from 2 µL to 1 mL
• Deionized (DI) water
• Coating Buffer: 8.4 g NaHCO₃, 3.56 g Na₂CO₃, add DI H₂O to 1.0 L, pH to 9.5 (BioLegend Cat. No. 421701 is recommended.)
• Assay Diluent: 10% Fetal Bovine Serum or 1% BSA in Phosphate-Buffered Saline (PBS) (BioLegend Cat. No. 421203 is recommended.)
• PBS: 8.0 g NaCl, 1.16 g Na₂HPO₄, 0.2 g KH₂PO₄, 0.2 g KCl, add DI water to 1.0 L, pH to 7.4
• Wash Buffer: Phosphate-Buffered Saline (PBS) + 0.05% Tween-20 (BioLegend Cat. No. 421601 is recommended.)
• Wash bottle or automated microplate washer
• TMB Substrate Solution – BioLegend Cat. No. 421101 is recommended.
• Stop Solution (2 N H₂SO₄)
• Log-Log graph paper or software for data analysis
• Tubes to prepare standard dilutions
• Timer
• Absorbent paper

Kit Contents

• Mouse IL-4 ELISA MAX™ Capture Antibody (200X)
• Mouse IL-4 ELISA MAX™ Detection Antibody (200X)
• Mouse IL-4 Standard
• Avidin-HRP (1000X)

Cell Sources

Mast cells, T cells, bone marrow stromal cells

Gene ID

16189View all products for this Gene ID

UniProt

View information about IL-4 on UniProt.org