Mouse IL-1b ELISA MAX™ Deluxe 20 Plates | Medical Supply Company

Mouse IL-1b ELISA MAX™ Deluxe 5 Plates

SKU: 432604 Categories: Deluxe Set, ELISA, Mouse, Sandwich Tag: Biolegend BV

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Mouse IL-1b ELISA MAX™ Deluxe 5 Plates

ELISA Development Systems Engineered for Sensitive, Precise and Reliable Serum and Plasma Cytokine Quantitation

BioLegend’s ELISA Max™ Sets provide all the time-savings, convenience and performance of a high-end, commercial ELISA kit for a fraction of the price! BioLegend’s ELISA Max™ Sets, in two appealing formats, address the need for sensitivity, accuracy, dependability, and versatility of an ELISA development system, while providing maximum cost effectiveness, value, and ease of use.

Both the ELISA Max™ Set Standard and ELISA Max™ Set Deluxe are specifically engineered for accurate recovery and measurement of analytes in complex fluids, such as serum and plasma (as well as activated cell cultures) without any interference by confounding serum factors, such as heterophilic antibody or rheumatoid factor.

Reactivity

Mouse

Application

ELISA

As a part of BioLegend's efforts to go green, each new lot of the ELISA MAX™ sets will include a summarized version of its manual. Click here for more information.

Application Notes

BioLegend’s mouse and human IL-1β ELISA kits use antibodies raised against the mature form of IL-1β. However, because pro-IL-1β contains the mature form, these antibodies likely recognize both the pro- and the mature forms of the protein. The human IL-1β ELISA kits have been shown to equally recognize both pro- and mature forms of IL-1β.

Application References (PubMed link indicates BioLegend citation)

O'Sullivan BJ, et al. 2006. J. Immunol. 176:7278.
Huck O, et al. 2014. Innate Immun. 21:65. PubMed
Alves BN, et al. 2014. J. Immunol. 192:3121. PubMed
Haldar S, et al. 2015. J Biol Chem. 290:6574. PubMed
Huck O, et al. 2014. Innate Immunity. 21:65. PubMed
Alves B, et al. 2014. J Immunol. 192:3121. PubMed
Haldar S, et al. 2015. J Biol Chem. 290:6574. PubMed
Zeis T, et al. 2015. Brain Behav Immun. 48: 313-325. PubMed
Schaafsma W, et al. 2015. Brain Behav Immun. 48: 205-221. PubMed
Naji A, et al. 2016. Sci Rep. 6: 26162. PubMed
Shih S, et al. 2016. PLoS One. 11: 0157647. PubMed

Sensitivity

16 pg/mL

Standard Range

31.3-2,000 pg/mL

Materials Not Included

A microplate reader capable of measuring absorbance at 450 nm
Adjustable pipettes to measure volumes ranging from 2 µL to 1 mL
Deionized (DI) water
PBS (Phosphate-Buffered Saline): 8.0 g NaCl, 1.16 g Na₂HPO₄, 0.2 g KH₂PO₄, 0.2 g KCl, add deionized water to 1 L; pH to 7.4, 0.2 µm filtered.
Wash Buffer (Phosphate-Buffered Saline (PBS) + 0.05% Tween-20, pH=7.4). BioLegend Cat. No. 421601 is recommended.
Wash bottle or automated microplate washer
Stop Solution (e.g. 2N H₂SO₄) Biolegend Cat. No. 423001 is recommended
Log-Log graph paper or software for data analysis
Tubes to prepare standard dilutions
Timer
Plate Sealer
Absorbent paper

Kit Contents

Mouse IL-1β ELISA MAX™ Capture Antibody (200X)
Mouse IL-1β ELISA MAX™ Detection Antibody (200X)
Mouse IL-1β Standard
Avidin-HRP (1000X)
Substrate Solution D
Coating Buffer A (5X)
Assay Diluent A (5X)
NUNC Maxisorp™ 96 MicroWell Plates

Cell Sources: Monocytes, tissue macrophages, Langerhan cells, dendritic cells, T lymphocytes, B lymphocytes, natural killer (NK) cells, large granular lymphocytes (LGL), vascular endothelium, smooth muscle, fibroblasts, thymic epithelia, astrocytes, microglia, glioma
Gene ID: 16176View all products for this Gene ID
UniProt: View information about IL-1beta on UniProt.org