PCRBIO Rapid Extract PCR Kit

Description

Direct PCR from mammalian tissue

PCRBIO Rapid Extract PCR Kit has been developed for fast, efficient amplification of DNA from a variety of tissues and is particularly suited to solid tissue such as mouse tail or mouse ear. Sample processing is simplified and contamination risks minimised as DNA extraction is performed in a single tube, removing the need for multiple washing steps.

Extracted DNA is amplified in a proprietary buffer system using PCRBIO HS Taq Mix Red. Our antibody-mediated hot start polymerase uses the latest developments in polymerase technology and buffer chemistry to enhance PCR speed, yield and sensitivity. Primer dimer formation and non-specific amplification are prevented giving superior specificity ideal for complex templates such as mammalian genomic DNA. The mix contains a red dye suitable for direct gel loading without the need for additional loading buffer.

Features

• Rapid, convenient, single-tube DNA extraction
• Produces high yield, PCR-ready DNA in as little as 15 minutes
• Maximised PCR speed, yield and specificity
• Powered by PCRBIO HS Taq Mix Red for direct gel loading – saving you even more time
• Ideal for complex templates
• Suitable for multiple sample types including mouse tail clip and ear punch, animal tissue, hair follicle, buccal swab, mammalian blood and FFPE tissue

Applications

• Genotyping
• Transgene detection
• Knockout analysis
• Sequencing

Size

400 reactions