UltraScript Reverse Transcriptase

Description

cDNA Synthesis

UltraScript Reverse Transcriptase is a thermostable and extremely active modified MMLV reverse transcriptase (RTase). The reaction temperature can be increased up to 55°C providing higher specificity and efficient transcription of GC-rich RNA regions with a high secondary structure. The RTase is blended with an advanced RNase inhibitor preventing degradation of RNA by contaminating RNase. UltraScript Reverse Transcriptase is not inhibited by ribosomal and transfer RNAs, making total RNA an ideal substrate.

The enzyme is supplied with a 5x buffer containing Mg, dNTPs, stabilizers and enhancers, optimised to generate high yield cDNA from as little as 4pg RNA. As oligos are not included, UltraScript Reverse Transcriptase provides the convenience and flexibility for users to define their own priming strategy depending on the type of analysis needed. UltraScript Reverse Transcriptase gives exceptional performance with gene-specific primers, oligo(dT) and random hexamers to produce high quality cDNA ideal for a variety of downstream applications.

Features

• Recommended product for reverse transcription step of COVID-19 diagnostic tests
• Sensitive and accurate reverse transcription of viral RNA sequences, including SARS-CoV-2
• Thermostable reverse transcriptase 45°C to 55°C
• Advanced RNase inhibitor
• High cDNA yields from as little as 4pg total RNA
• Accurate reverse transcription of GC-rich templates
• Sensitive detection of low copy number transcripts
• Advanced buffer chemistry including Mg and dNTPs

Applications

• Random hexamer, oligo(dT) and gene-specific primers
• cDNA synthesis for PCR analysis, cloning, library preparation and Next Generation Sequencing
• Low copy number transcripts
• Viral RNA targets
• miRNA targets
• Efficient synthesis from total RNA or poly(A)+ RNA

Size

10,000 units