Venor®GeM Classic

Venor®GeM Classic

Description

Venor®GeM Classic allows fast, reliable and time-saving routine monitoring of mycoplasma contamination in cell culture supernatants, media and biopharmaceuticals in research and industry by conventional PCR.
All Mollicutes (Mycoplasma, Acholeplasma, Spiroplasma) species so far described as contaminants of cell cultures and media components are specifically detected by amplification of a highly conserved rRNA operon, or more specifically, of the 16S rRNA coding region in the mycoplasma genome. The mycoplasma-specific amplification is detected at 265-278 bp. False negative results due to PCR inhibitors or improper DNA extraction can be ruled out by using the provided internal amplification control, which is detected at 191 bp.

Recommended Use

Applicable in research and industry for the direct detection of Mollicutes (Mycoplasma, Acholeplasma, Spiroplasma) contaminations in cell cultures and biopharmaceuticals. Approved for testing according to EP 2.6.7 and JP G3. Intended for research use only. Not recommended for clinical diagnostics.

Kit Components

Lyophilized Mix: Primer sets / Nucleotides in aliquots of 25 reactions each
10x Reaction Buffer (optimized for MB Taq DNA Polymerase)
Lyophilized Positive Control DNA
Lyophilized Internal Control DNA
PCR Grade Water

Required Consumables

Taq DNA Polymerase. We highly recommend our reliable hot-start MB Taq DNA Polymerase, Cat. No. 53-0050/-0100/-0200/-0250 or 53-1050/-1100/-1200/-1250.
PCR reaction tubes

Required Lab Devices

PCR cycler for conventional PCR
Agarose gel electrophoresis and DNA staining system
Pipetting equipment
Tube centrifuge

Shelf Life and Storage

Store the unopened components at +2 °C to +8 °C until the expiry date indicated on the label. Once rehydrated, the components must be stored at ≤ -18 °C.