Flex-T™ HLA Class I ELISA Control 20 ug
The Flex-T™ system is designed to provide maximal flexibility in the study of antigen specific T cells. Flex-T™ Monomer UVX products are a biotinylated HLA class I α-chain, associated with the β2-microglobulin chain, and stabilized by a UV-labile peptide. The C-terminus of the α-chain is biotinylated by the enzyme BirA. In the absence of proper peptide binding, the HLA class I complex quickly disassociates. Thus, by using UV radiation, it is possible to exchange the labile peptide with another peptide of interest. An ELISA was designed to verify this exchange process. A 96-well plate coated with Streptavidin captures the biotinylated α-chain, followed by detection of the associated β2-microglobulin with HRP-conjugated anti-β2-microglobulin antibody. The Flex-T™ HLA Class I ELISA Control is a HLA class I α-chain associated with β2-microglobulin and stabilized with a high-affinity, non UV-labile peptide. This stable monomer serves as positive control for this ELISA.
- PBS (pH 7.5) containing 0.5% BSA, protease inhibitors, 0.05% sodium azide.
- 0.2 mg/ml
- Storage & Handling
- Store frozen (-20 or -80°C); shipped in blue ice. Aliquot upon receipt before freezing; avoid repeated freeze-thaw cycles.
ELISA – Quality tested
FC – Validated
- Recommended Usage
Recommended final concentrations as positive controls are 2.7nM, 1.4nM, and 0.7nM for HLA Class I ELISA. Use 5 μl to start the dilutions as described in the application notes.
- Application Notes
Take 5 μl of the solution and dilute it into 7.88ml of assay dilution buffer. This will result in the High control for the HLA class I ELISA (2.7nM). 2X serial dilutions with assay dilution buffer will result in the Medium (1.4nM) and Low (0.7nM) controls. This amount is sufficient for up to 15 plates. However, it is recommended that ELISA controls be prepared freshly for each assay.
To order custom products please fill out the Custom Flex-T™ Request Form.
- Application References
(PubMed link indicates BioLegend citation)
- Altman JD, et al. 1996. Science 274:94-6.
- Rodenko B, et al. 2006. Nat. Protoc. 1:1120.
- Toebes M, et al. 2006. Nat. Med. 12:246.
- Bakker AH, et al. 2008. Proc. Natl. Acad. Sci. USA 105:3825.
- Gene ID