LEGEND MAX™ Human GM-CSF ELISA Kit with Pre-coated Plates 1 Pre-coated Plate
Granulocyte/macrophage – colony stimulating factor (GM-CSF) is a hematopoietic factor that is produced by activated T cells, B cells, mast cells, macrophages, fibroblasts and endothelial cells. In addition to supporting colony formation of granulocyte/macrophage progenitors, GM-CSF is a growth factor for erythroid, megakaryocyte and eosinophil progenitors.
BioLegend’s LEGEND MAX™ Human GM-CSF ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay (ELISA), in which, a Human GM-CSF specific monoclonal antibody is pre-coated on a 96-well strip-well plate.
BioLegend’s LEGEND MAX™ Human GM-CSF ELISA Kit is specifically designed for the accurate quantitation of Human GM-CSF from cell culture supernatant, serum, plasma or other bodily fluids. It is ready-to-use, accurate, and sensitive.
- Reactivity
- Human
- Application
ELISA
- Application References
(PubMed link indicates BioLegend citation)
- Samavedam UK, et al. 2014. J. Immunol. 192:559. PubMed
- Samavedam U, et al. 2014. J Immunol. 192:559. PubMed
- Sensitivity
- 3.5 pg/mL
- Standard Range
- 15.6-1,000 pg/mL
- Materials Not Included
- Microplate reader able to measure absorbance at 450 nm
- Adjustable pipettes to measure volumes ranging from 1 µL to 1,000 µL
- Deionized water
- Wash bottle or automated microplate washer
- Log-Log graph paper or software for data analysis
- Tubes to prepare standard dilutions
- Timer
- Plate Shaker
- Polypropylene vials
- Kit Contents
- Anti-Human GM-CSF Pre-coated 96-well Strip Microplate
- Human GM-CSF Dectection Antibody
- Human GM-CSF Standard
- Matrix A
- Avidin-HRP A
- Assay Buffer A
- Wash Buffer (20X)
- Substrate Solution F
- Stop Solution
- Plate Sealers
- Cell Sources
- T cells, macrophages, fibroblasts, endothelial cells, mast cells
- Gene ID
- NA
- UniProt
- View information about GM-CSF on UniProt.org
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