LEGEND MAX™ Mouse IL-21 ELISA Kit with Pre-coated Plates
Interleukin 21 (IL-21) is a cytokine produced by CD4+ T cells, including T helper 17 (Th17) cells, T follicular helper (Tfh) cells, and natural killer T (NKT) cells. Functionally, IL-21 has pleiotropic effects on both innate and adaptive immune responses. IL-21 enhances IFNγ production and cytotoxicity in CD8+ T effector cells, and terminal differentiation in activated natural killer (NK) cells. Though not essential for the differentiation of Th17 cells in vitro or in vivo, IL-21 can drive Th17 responses in conjunction with TGF-β. Production of IL-21 in CD4+ T cells is induced by IL-6 upon TCR stimulation; IL-6 and IL-21 are also key players in Tfh differentiation, driving increased expression of both bcl6 and CXCR5. In addition to its effects on T cells, IL-21 can also mediate cell proliferation, growth arrest, terminal differentiation, and apoptosis in normal B cells, depending on the target cell’s activation status. However, IL-21 has been reported to inhibit the proliferation of both human and murine B cells that have been stimulated with anti-IgM antibodies.
The BioLegend LEGEND MAX™ Mouse IL-21 ELISA Kit is a Sandwich Enzyme-Linked Immunosorbent Assay (ELISA) with a 96-well strip plate that is pre-coated with a polyclonal goat anti-mouse IL-21 capture antibody. The detection antibody is a biotinylated polyclonal goat anti-mouse IL-21 antibody. This kit is specifically designed for the accurate quantitation of mouse IL-21 from cell culture supernatant, serum, plasma, and other biological fluids. It is analytically validated with ready-to-use reagents.
- Reactivity
- Mouse
- Storage & Handling
- Store unopened kit components between 2°C and 8°C.
- Application
ELISA
- Sensitivity
- 1.39 pg/mL
- Standard Range
- 4.7-300 pg/mL
- Materials Not Included
- Microplate reader able to measure absorbance at 450 nm
- Adjustable pipettes to measure volumes ranging from 1 µL to 1,000 µL
- Deionized water
- Wash bottle or automated microplate washer
- Log-Log graph paper or software for data analysis
- Tubes to prepare standard dilutions
- Timer
- Plate Shaker
- Polypropylene vials
- Kit Contents
- Anti-Mouse IL-21 Pre-coated 96-well Strip Microplate
- Mouse IL-21 Dectection Antibody
- Mouse IL-21 Standard
- Matrix C (for serum and plasma samples only)
- Avidin-HRP
- Assay Buffer A
- Wash Buffer (20X)
- Substrate Solution F
- Stop Solution
- Plate Sealers
- Cell Sources
- Th17, follicular helper T cells (TFH), Th2, memory CD4+ T cell, and NKT cells.
- Gene ID
- 60505View all products for this Gene ID
- UniProt
- View information about IL-21 on UniProt.org
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