Human alpha-Synuclein ELISA Kit 1 kit
α-synuclein, Alpha-synuclein, is expressed principally in the central nervous system (brain) but is also expressed in low concentrations in a variety of tissues except liver. It is predominantly expressed in the neocortex, hippocampus, substantia nigra, thalamus, and cerebellum of the CNS. It is primarily a neuronal protein, but can also be found in the neuroglial cells. It is concentrated in presynaptic nerve terminals of neurons, as well as having reported nuclear and mitochondrial localization. α-synuclein interacts with plasma membrane phospholipids. α-synuclein in solution is considered to be an intrinsically disordered protein and thus lacks a stable secondary or tertiary structure. However, recent data suggests the presence of partial alpha helical as well as beta sheet structures as well as mostly structured tetrameric states in solution, the equilibrium of which may be altered by binding partners. The human α-synuclein protein is made of 140 amino acids, encoded by the SNCA gene. The primary structure is divided in three distinct domains: (1-60) – An amphipathic N-terminal region dominated by four 11-residue repeats including the consensus sequence KTKEGV. This sequence has a structural alpha helix propensity similar to apolipoproteins-binding domains. (61-95)- a central hydrophobic region which includes the non-amyloid-β component (NAC) region, involved in protein aggregation. (96-140)- a highly acidic and proline-rich region. At least three isoforms of synuclein are produced through alternative splicing. The most common form of the protein, is the full 140 amino acid-long transcript. Other isoforms are alpha-synuclein-126, lacking residues 41-54; and α-synuclein-112, which lacks residues 103-130. α-synuclein may be involved in the regulation of dopamine release and transport and also may function to induce fibrillization of microtubule-associated protein tau. α-synuclein functions as a molecular chaperone in the formation of SNARE complexes. In particular, it can bind to phospholipids of the plasma membrane and to synaptobrevin-2 via its C-terminus domain to influence synaptic activity. α-synuclein is essential for normal development of the cognitive functions and that it significantly interacts with tubulin. It also reduces neuronal responsiveness to various apoptotic stimuli, leading to decreased caspase-3 activation. α-Synuclein fibrils are major substituent of the intracellular Lewy bodies seen in Parkinson’s disease.
- Specific for human α-synuclein.
- Storage & Handling
- Store between 2°C and 8°C.
- Recommended Usage
Please download the LEGEND MAX™ Human α-Synuclein ELISA Kit Protocol for further assay detail.
- Application Notes
This kit has a guaranteed shelf life of 2 to 6 months.
Please note that the name of this kit has changed from Human α-Synuclein ELISA Kit to LEGEND MAX™ Human α-Synuclein ELISA Kit with Pre-coated Plate.
- Application References
(PubMed link indicates BioLegend citation)
- Kang JH, Irwin DJ, Chen-Plotkin AS, Siderowf A; et al. Association of cerebrospinal fluid β-Amyloid 1-42, T-tau, P-tau181, and α-Synuclein levels with clinical features of drug-naive patients with early Parkinson disease. JAMA Neurology, Aug 2013.
- Mondello S, Buki A, Italiano D and Jeromin A. α-Synuclein in CSF of patients with severe traumatic brain injury. Neurology: 80: 1662-1668, Apr 2013.
- Mollenhauer B, Trautmann E, Taylor P, Manninger P, Sixel-D÷ring F, Ebentheuer J, Trenkwalder C, Schlossmacher M. Total α-synuclein is lower in de novo Parkinson patients than in healthy subjects. Neuroscience: 532: 44-484, Jan 2013.
- Oeckl P, et al. 2016. Mol Cell Proteomics. 15: 3126 – 3138. PubMed
- Eidson L, et al. 2017. J Neuroinflammation.. 10.1186/s12974-017-0935-1. PubMed
- Mollenhauer B, et al. 2017. Mov Disord.. 10.1002/mds.27090. PubMed
- Mollenhauer B, et al. 2016. Neurology. 10.1212/WNL.0000000000002651. PubMed
- Kang J, et al. 2016. Acta Neuropathol.. 10.1007/s00401-016-1552-2. PubMed
- Kruse N, et al. 2015. Neurobiol Aging. 10.1016/j.neurobiolaging.2015.05.003. PubMed
- Standard Range
- 6.1-1500 pg/mL
- Materials Not Included
- Microplate reader
- Adjustable pipettes to measure volumes ranging from
- Deionized water
- Wash bottle or automated microplate washer
- Log-Log graph paper or software for data analysis
- Tubes to prepare standard dilutions
- Plate Shaker
- Polypropylene vials
- Kit Contents
- Capture Antibody Coated Plate
- α-Synuclein Standard
- 5X Wash Buffer
- 2X Reagent Diluent
- Biotinylated Primary Antibody
- Chemiluminescent Substrate A
- Chemiluminescent Substrate B
- Plate Sealer
- Gene ID
- 6622View all products for this Gene ID
- View information about alpha-Synuclein on UniProt.org