LEGEND MAX™ Human Erythropoietin (EPO) ELISA Kit with Pre-coated Plates 1 Pre-coated Plate

Erythropoietin (EPO) is the most important hormone regulating erythropoiesis. It is a glycoprotein also known as hematopoietin. Human EPO is mainly produced by kidney and liver in response to hypoxia to induce red blood cell synthesis. EPO is a secreted protein of 166 amino acids with a predicted molecular mass of ~18 kD. However, it migrates on SDS-PAGE at ~35-55 kD due to heavy glycosylation. Human EPO is closely related to thrombopoietin (TPO), sharing 44% amino acid homology. EPO is highly conserved across species, with human EPO being 80% identical to mouse, and mouse EPO being 95% identical to rat.

EPO functions through binding to its receptor, EPO R, a homodimer, on erythoid precursors, and promotes their differentiation and maturation. As expected, serum levels of EPO are elevated in response to various conditions that result in tissue hypoxia (e.g. blood loss, high altitude, cardiac or pulmonary disease, and anemia). Increased EPO levels can also be due to increased production by cancer cells of the liver and kidney. Decreased levels of EPO are often seen with chronic renal failure and other chronic diseases or chemotherapy, whcih cause anemia. Human endogenous EPO and recombinant EPO share the same amino acid sequence, with differences in the glycosylation profiles.

The LEGEND MAX™ Human EPO ELISA kit is a Sandwich Enzyme-Linked Immunosorbent Assay (ELISA) with a 96-well strip plate that is pre-coated with a mouse monoclonal anti-human EPO antibody. The Detection Antibody is a biotinylated mouse monoclonal anti-human EPO antibody. This kit is specifically designed for the accurate quantitation of human EPO from cell culture supernatant, serum, plasma (heparin, EDTA and citrate), urine, and other biological fluids. This kit is analytically validated with ready-to-use reagents.