LEGEND MAX™ Mouse IL-9 ELISA Kit with Pre-coated Plates 5 Pre-coated Plates
Mouse Interleukin 9 (IL-9) is a 144 amino acid, 16.1 kD member of the Th2 cytokine family. Initially, IL-9 was identified in mice as a T-cell growth factor. Mouse IL-9 shares 56% and 75% amino acid identity with human IL-9 and rat IL-9, respectively.
IL-9 is a pleiotropic cytokine that is involved in cell proliferation, inflammation, and apoptosis. It has biological effects on hematopoietic progenitor cells, lymphocytes, mast cells, airway epithelial cells and smooth muscle cells. IL-9 functions through binding to the IL-9 receptors which consists of the α-chain (IL-9Rα) and the common γ-chain that is shared by IL-2, IL-4 and IL-7 cytokines. When IL-9 binds to its receptors, the receptor complex activates the STAT signaling pathway, thus connecting this cytokine to various biological processes. In addition, IL-9 is produced by activated Th9, Th17, Treg cells and appears to be a key factor in the development and function of subsets within the CD4+ T cell lineage. Experiments on a mouse model of asthma demonstrates that IL-9 plays an important role in allergic inflammation. It has been described that IL-9 and IL-9R expression are increased in lungs of asthmatic patients. In addition, IL-9 has been identified to promote the production of mast cells in the intestinal and respiratory epithelia. These characteristics make IL-9 a target of interest in studies of hypersensitivity disorders such as asthma and other diseases.
BioLegend’s LEGEND MAX™ Mouse IL-9 Sandwich Enzyme-Linked Immunosorbent Assay (ELISA) kit includes a 96-well strip plate that is pre-coated with a rat monoclonal anti-mouse IL-9 capture antibody. The detection antibody is a biotinylated goat polyclonal anti-mouse IL-9 antibody. This kit is specifically designed for the accurate quantitation of mouse IL-9 from cell culture supernatant, serum, and plasma. It is analytically validated with ready-to-use reagents.
- Reactivity
- Mouse
- Application
ELISA
- Sensitivity
- 0.258 pg/mL ± 0.092 pg/mL
- Standard Range
- 1.56 – 100 pg/mL
- Materials Not Included
- Microplate reader able to measure absorbance at 450 nm
- Adjustable pipettes to measure volumes ranging from 1 µL to 1,000 µL
- Deionized water
- Wash bottle or automated microplate washer
- Log-Log graph paper or software for data analysis
- Tubes to prepare standard dilutions
- Timer
- Plate Shaker
- Polypropylene vials
- Kit Contents
- Anti-Mouse IL-9 Pre-coated 96-well Strip Microplate
- Mouse IL-9 Detection Antibody
- Mouse IL-9 Standard
- Avidin-HRP
- Assay Buffer A
- Wash Buffer (20X)
- Substrate Solution F
- Stop Solution
- Plate Sealers
- Gene ID
- 16198View all products for this Gene ID
- UniProt
- View information about IL-9 on UniProt.org





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