LEGEND MAX™ Mouse IL-9 ELISA Kit with Pre-coated Plates 5 Pre-coated Plates

Mouse Interleukin 9 (IL-9) is a 144 amino acid, 16.1 kD member of the Th2 cytokine family. Initially, IL-9 was identified in mice as a T-cell growth factor.  Mouse IL-9 shares 56% and 75% amino acid identity with human IL-9 and rat IL-9, respectively.

IL-9 is a pleiotropic cytokine that is involved in cell proliferation, inflammation, and apoptosis. It has biological effects on hematopoietic progenitor cells, lymphocytes, mast cells, airway epithelial cells and smooth muscle cells.  IL-9 functions through binding to the IL-9 receptors which consists of the α-chain (IL-9Rα) and the common γ-chain that is shared by IL-2, IL-4 and IL-7 cytokines. When IL-9 binds to its receptors, the receptor complex activates the STAT signaling pathway, thus connecting this cytokine to various biological processes. In addition, IL-9 is produced by activated Th9, Th17, Treg cells and appears to be a key factor in the development and function of subsets within the CD4⁺ T cell lineage. Experiments on a mouse model of asthma demonstrates that IL-9 plays an important role in allergic inflammation. It has been described that IL-9 and IL-9R expression are increased in lungs of asthmatic patients. In addition, IL-9 has been identified to promote the production of mast cells in the intestinal and respiratory epithelia. These characteristics make IL-9 a target of interest in studies of hypersensitivity disorders such as asthma and other diseases.

BioLegend’s LEGEND MAX™ Mouse IL-9 Sandwich Enzyme-Linked Immunosorbent Assay (ELISA) kit includes a 96-well strip plate that is pre-coated with a rat monoclonal anti-mouse IL-9 capture antibody. The detection antibody is a biotinylated goat polyclonal anti-mouse IL-9 antibody. This kit is specifically designed for the accurate quantitation of mouse IL-9 from cell culture supernatant, serum, and plasma. It is analytically validated with ready-to-use reagents.