Mouse IL-9 ELISA MAX™ Deluxe 5 Plates
Mouse Interleukin 9 (IL-9) is a 144 amino acid, 16.1 kD member of the TH2 cytokine family. Originally identified as a mast cell growth factor, IL-9 has been identified to promote IL-6 and protease production in mast cells that can contribute to the severity of hypersensitivity disorders. These characteristics, among others, have made IL-9 a molecule of interest in studies of hypersensitivity disorders, such as asthma.
BioLegend’s ELISA MAX™ Deluxe Set is a sandwich Enzyme-Linked Immunosorbent Assay (ELISA). A mouse IL-9 specific rat monoclonal antibody is first coated on a 96-well plate. Standards and samples are added to the wells, and IL-9 binds to the immobilized capture antibody. Next, a biotinylated goat polyclonal anti-mouse IL-9 detection antibody is added, producing an antibody-antigen-antibody “sandwich”. Avidin-horseradish peroxidase is subsequently added, followed by TMB Substrate Solution, producing a blue color in proportion to the concentration of IL-9 present in the sample. Finally, the Stop Solution changes the reaction color from blue to yellow, and the microwell absorbance is read at 450 nm with a microplate reader.
- Reactivity
- Mouse
- Application
ELISA
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- Sensitivity
- 1.8 pg/mL
- Standard Range
- 3.2 – 200 pg/mL
- Materials Not Included
- PBS (Phosphate-Buffered Saline): 8.0 g NaCl, 1.16 g Na2HPO4, 0.2 g KH2PO4, 0.2 g KCl, add deionized water to 1 L; pH to 7.4, 0.2 μm filtered.
- Wash Buffer: PBS + 0.05% Tween-20, BioLegend Cat. No. 421601 is recommended.
- Stop Solution: BioLegend Cat. No. 423001 is recommended, or acid solution, e.g. 2N H2SO4
- Plate Sealers: BioLegend Cat. No. 423601 is recommended.
- Deionized (DI) water
- A microplate reader capable of measuring absorbance at 450 nm
- Adjustable pipettes to measure volumes ranging from 2 μL to 1 mL
- Wash bottle or automated microplate washer
- Log-Log graph paper or software for data analysis
- Tubes to prepare standard dilutions
- Timer
- Absorbent paper
- Kit Contents
- Mouse IL-9 ELISA MAX™ Capture Antibody (200X)
- Mouse IL-9 ELISA MAX™ Detection Antibody (200X)
- Mouse IL-9 Standard
- Avidin-HRP (1000X)
- Substrate Solution F
- Coating Buffer A (5X)
- Assay Diluent A (5X)
- NUNC Maxisorp™ 96 MicroWell Plates
- Lot-Specific Certificate of Analysis/ELISA MAX™ Deluxe Set Protocol
- Gene ID
- 16198View all products for this Gene ID
- UniProt
- View information about IL-9 on UniProt.org
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