LEGEND MAX™ Mouse NGAL (Lipocalin-2) ELISA Kit with Pre-coated Plates 1 Pre-coated Plate
Neutrophil Gelatinase-Associated Lipocalin (NGAL), also known as Lipocalin-2, Siderocalin, 24p3, Uterocalin, or Neu-related Lipocalin, is a member of the lipocalin family. It is a secreted protein (25kD), which captures bacterial siderophores that bind iron with high affinity. Studies with human samples show that NGAL predominantly exists in monomeric form, with a small portion in homodimeric and homotrimeric forms. It can also form a heterodimer with MMP-9 and a heterotrimer with MMP-9 and TIMP-1. Mouse NGAL shares 62% and 80% amino acid sequence identity with human and rat, respectively.
The functions of NGAL are diverse and continue to be studied. NGAL acts as a potent anti-bacterial agent via siderophore-mediated iron depletion. NGAL knockout mice exhibit decreased ability to combat bacterial infection. NGAL has been used as an early predictive and prognostic biomarker for acute and chronic kidney injuries. It has been associated with breast, lung, colon, and pancreatic cancer, having diverse, context-dependent effects during tumor development. This may involve cell growth, survival, migration, invasion, and angiogenesis. When NGAL forms a complex with MMP-9, it protects MMP-9 from degradation, thereby preserving MMP-9’s enzymatic activity, which has been involved in the development of several types of cancers.
The LEGEND MAX™ Mouse NGAL ELISA kit is a Sandwich Enzyme-Linked Immunosorbent Assay (ELISA) with a 96-well strip plate that is pre-coated with rat monoclonal anti-mouse NGAL antibody. The Detection Antibody is a biotinylated rat monoclonal anti-mouse NGAL antibody. This kit is specifically designed for the accurate quantitation of mouse NGAL from cell culture supernatant, cell lysate, serum, plasma, urine, and other biological fluids. This kit is analytically validated with ready-to-use reagents.
- Reactivity
- Mouse
- Application
ELISA
- Sensitivity
- 22.5 pg/mL
- Standard Range
- 78 – 5,000 pg/mL
- Materials Not Included
- Microplate reader able to measure absorbance at 450 nm
- Adjustable pipettes to measure volumes ranging from 1 µL to 1,000 µL
- Deionized water
- Wash bottle or automated microplate washer
- Log-Log graph paper or software for data analysis
- Tubes to prepare standard dilutions
- Timer
- Plate Shaker
- Polypropylene vials
- Kit Contents
- Anti-Mouse NGAL Pre-coated 96 well Strip Microplate
- Mouse NGAL Detection Antibody
- Mouse NGAL Standard
- Avidin-HRP
- Assay Buffer B
- Wash Buffer (20X)
- Substrate Solution D
- Stop Solution
- Plate Sealers
- Gene ID
- 16819View all products for this Gene ID
- UniProt
- View information about NGAL on UniProt.org
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