LEGEND MAX™ Mouse CXCL13 (BLC) ELISA Kit with Pre-coated Plates 1 Pre-coated Plate
CXCL13, also known as B-Lymphocyte Chemoattractant (BLC), or B Cell-Attracting chemokine 1 (BCA-1), is a small cytokine belonging to the CXC chemokine family. As a strong chemoattractant for B cells, it is highly expressed in the follicles of Peyer’s patches, spleen, and lymph nodes. It is also expressed by T follicular helper cells, dendritic cells, and stromal cells (including marginal reticular cells (MRCs) and follicular dendritic cells (FDCs)) in B cell areas of secondary lymphoid tissue. CXCL13 and its receptor CXCR5 regulate compartmentalization of B- and T-cells in secondary lymphoid organs. During the migration in the follicle, B cells are in contact with FDCs, MRCs, and cells localized around the follicle perimeter, such as sinus-associated macrophages and T cell zone associated DCs. Subsequent to the migration through the FDC network, B cells travel through the T cell zone-proximal follicle. In this migration, CCL21, CCL19, CCR7 and CXCR5 are involved. CXCL13, CCL19, and CCL21 take part in the formation of tertiary lymphoid tissues in chronic antigen-induced arthritis. In CXCL13 deficient mice, B-cells fail to organize in polarized follicular clusters and instead appeare as a ring of cells at the perimeter of T-cell areas.
CXCL13 is highly expressed at sites of new lymphoid tissue formation in a variety of chronic inflammatory conditions. High expression of CXCL13 has been linked to lupus, Helicobacter pylori-associated gastritis, multiple sclerosis, rheumatoid arthritis, and the allergic airway inflammatory process.
LEGEND MAX™ Mouse CXCL13 (BLC) ELISA kit is a Sandwich Enzyme-Linked Immunosorbent Assay (ELISA) with a 96-well strip plate that is pre-coated with a rat monoclonal anti-mouse CXCL13 antibody. The detection antibody is a biotinylated rat monoclonal anti-mouse CXCL13 antibody. This kit is specifically designed for the accurate quantitation of mouse CXCL13 from cell culture supernatant, serum and plasma. This kit is analytically validated with ready-to-use reagents.
- Reactivity
- Mouse
- Application
ELISA
- Sensitivity
- 1.0 ± 0.4 pg/mL
- Standard Range
- 7.8 – 500 pg/mL
- Materials Not Included
- Microplate reader able to measure absorbance at 450 nm
- Adjustable pipettes to measure volumes ranging from 1 µL to 1,000 µL
- Deionized water
- Wash bottle or automated microplate washer
- Log-Log graph paper or software for data analysis
- Tubes to prepare standard dilutions
- Timer
- Plate Shaker
- Polypropylene vials
- Kit Contents
- Anti-Mouse CXCL13 Precoated 96-well Strip Microplate
- Mouse CXCL13 Detection Antibody
- Mouse CXCL13 Standard
- Avidin-HRP
- Assay Buffer B
- Wash Buffer (20X)
- Substrate Solution F
- Stop Solution
- Plate Sealers
- Gene ID
- 55985View all products for this Gene ID
- UniProt
- View information about CXCL13 on UniProt.org
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