Mouse IL-1b ELISA MAX™ Standard 5 Plates
ELISA Development Systems Engineered for Sensitive, Precise and Reliable Serum and Plasma Cytokine Quantitation
BioLegend’s ELISA Max™ Sets provide all the time-savings, convenience and performance of a high-end, commercial ELISA kit for a fraction of the price! BioLegend’s ELISA Max™ Sets, in two appealing formats, address the need for sensitivity, accuracy, dependability, and versatility of an ELISA development system, while providing maximum cost effectiveness, value, and ease of use.
Both the ELISA Max™ Set Standard and ELISA Max™ Set Deluxe are specifically engineered for accurate recovery and measurement of analytes in complex fluids, such as serum and plasma (as well as activated cell cultures) without any interference by confounding serum factors, such as heterophilic antibody or rheumatoid factor.
- Reactivity
- Mouse
- Application
ELISA
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- Application Notes
BioLegend’s mouse and human IL-1β ELISA kits use antibodies raised against the mature form of IL-1β. However, because pro-IL-1β contains the mature form, these antibodies likely recognize both the pro- and the mature forms of the protein. The human IL-1β ELISA kits have been shown to equally recognize both pro- and mature forms of IL-1β.
- Application References
(PubMed link indicates BioLegend citation)
- O'Sullivan BJ, et al. 2006. J. Immunol. 176:7278.
- Murayama MA, et al. 2014. Biochem Biophys Res Commun. 443:42. PubMed
- Rapsinski GJ, et al. 2015. Infect Immun. 83:693. PubMed
- Murayama M, et al. 2014. Biochem Biophys Res Commun. 443:42. PubMed
- Rapsinski G, et al. 2015. Infect Immun . 83:693. PubMed
- Brown P, et al. 2015. PLoS One. 10:125126. PubMed
- Andrade-Sousa A, et al. 2016. PLoS One. 11: 0163420. PubMed
- Th?nert R, et al. 2017. Nat Commun. 8:14268. PubMed
- Standard Range
- 31.3-2,000 pg/mL
- Materials Not Included
- Microwell plates: 96-well Nunc MaxiSorp™ is recommended.
- A microplate reader capable of measuring absorbance at 450 nm
- Adjustable pipettes to measure volumes ranging from 2 µL to 1 mL
- Deionized (DI) water
- Coating Buffer: 8.4 g NaHCO3, 3.56 g Na2CO3, add DI H2O to 1.0 L, pH to 9.5 (BioLegend Cat. No. 421701 is recommended.)
- Assay Diluent: 10% Fetal Bovine Serum or 1% BSA in Phosphate-Buffered Saline (PBS) (BioLegend Cat. No. 421203 is recommended.)
- PBS: 8.0 g NaCl, 1.16 g Na2HPO4, 0.2 g KH2PO4, 0.2 g KCl, add DI water to 1.0 L, pH to 7.4
- Wash Buffer: Phosphate-Buffered Saline (PBS) + 0.05% Tween-20 (BioLegend Cat. No. 421601 is recommended.)
- Wash bottle or automated microplate washer
- TMB Substrate Solution – BioLegend Cat. No. 421101 is recommended.
- Stop Solution (2 N H2SO4)
- Log-Log graph paper or software for data analysis
- Tubes to prepare standard dilutions
- Timer
- Absorbent paper
- Kit Contents
- Mouse IL-1β ELISA MAX™ Capture Antibody (200X)
- Mouse IL-1β ELISA MAX™ Detection Antibody (200X)
- Mouse IL-1β Standard
- Avidin-HRP (1000X)
- Cell Sources
- Monocytes, tissue macrophages, Langerhan cells, dendritic cells, T lymphocytes, B lymphocytes, natural killer (NK) cells, large granular lymphocytes (LGL), vascular endothelium, smooth muscle, fibroblasts, thymic epithelia, astrocytes, microglia, glioma
- Gene ID
- 16176View all products for this Gene ID
- UniProt
- View information about IL-1beta on UniProt.org
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