How do we validate our antibodies?
Knockout cell lines (cells with no or reduced expression of precise target proteins) is the new gold standard to validate the specificity of antibodies.
We have created 100s of CRISPR Knockout cell lines by introducing frame-shift mutations into the genomic DNA of target cells using sgRNA/CAS9.
The CRISPR knockout cells were lysed and Western Blotting was performed using Wild-Type (WT) cells as a control.
The Western Blots were then probed using our premium antibodies and visualisation of decreased protein expression was indicative of highly specific antibodies (Knockout Validated Antibodies).