IRIS Salmonella – Results on 1 plate in 37 Hours!

IRIS Salmonella – Results on 1 plate in 37 Hours | Medical Supply Company

IRIS Salmonella® is a rapid chromogenic method for detection of Salmonella spp. in human food, feeds and environmental samples. The method is AFNOR certified according to ISO 16140 standard for:

  • All human and animal food products and production and production environment samples (excluding primary production environment samples)
  • Detection of Salmonella spp. in samples from 50 g to 375 g for milk powder (including infant milk) with and without probiotics
  • Detection of Salmonella spp. in samples from 50 g to 125 g for flours and in animal feedstuffs.

IRIS Salmonella® Method

The full method uses Salmonella enrichment broth, a selective supplement and IRIS Salmonella agar which is available in dehydrated powder and pre-poured plates.

  • In the Enrichment step, the inoculum is added to Salmonella Enrichment medium and a selective IRIS Salmonella supplement is added.
  • After 18-hours incubation, plating can be performed on IRIS Salmonella Agar.
  • After just 24 hours the result is ready, with magenta colonies indicating presumptive Salmonella.

IRIS Salmonella® Agar can also be used in the standard ISO method as a second isolation medium.

Figure 1. Process flowchart for the detection of Salmonella spp. on IRIS agar

Increased flexibility

After incubation, the enrichment broth can be kept up to 3 days at 2-8°C before streaking onto IRIS Salmonella® Agar.

After incubation, the IRIS Salmonella® Agar plates may be kept up to 3 days at 2-8°C before reading and subsequent confirmations. 

Optimal detection of Salmonella spp.

The IRIS Salmonella media is very easy to use and provides an excellent contrast between agar and pink-magenta Salmonella colonies and the secondary flora, which appear blue to dark blue. Studies performed on IRIS Salmonella® Agar show a high specificity for the detection of Salmonellae including atypical species and serovars which are a source of confusion on other medium. The detection of typhoidal Salmonella, lactose-positive Salmonella and sucrose-positive strains is guaranteed. The agar allows the detection of non-motile serovars and monophasic strains as well as strains that show little to no esterase activity on other media.

How to confirm presumptive Salmonella?

  • Conventional tests described in standard methods CEN or ISO (including the purification step) using a colony isolated on IRIS Salmonella® Agar.
  • Latex test (CONFIRM Salmonella) made directly from a colony isolated on IRIS Salmonella® Agar

Figure 2. CONFIRM Salmonella latex test kits available from Biokar for the confirmation of presumptive Salmonella spp.

You can download the IRIS Salmonella Technical Datasheet for more information

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