IRIS Salmonella® is a rapid chromogenic method for detection of Salmonella spp. in human food, feeds and environmental samples. The method is AFNOR certified according to ISO 16140 standard for:
- All human and animal food products and production and production environment samples (excluding primary production environment samples)
- Detection of Salmonella spp. in samples from 50 g to 375 g for milk powder (including infant milk) with and without probiotics
- Detection of Salmonella spp. in samples from 50 g to 125 g for flours and in animal feedstuffs.
IRIS Salmonella® Method
The full method uses Salmonella enrichment broth, a selective supplement and IRIS Salmonella agar which is available in dehydrated powder and pre-poured plates.
- In the Enrichment step, the inoculum is added to Salmonella Enrichment medium and a selective IRIS Salmonella supplement is added.
- After 18-hours incubation, plating can be performed on IRIS Salmonella Agar.
- After just 24 hours the result is ready, with magenta colonies indicating presumptive Salmonella.
IRIS Salmonella® Agar can also be used in the standard ISO method as a second isolation medium.
After incubation, the enrichment broth can be kept up to 3 days at 2-8°C before streaking onto IRIS Salmonella® Agar.
After incubation, the IRIS Salmonella® Agar plates may be kept up to 3 days at 2-8°C before reading and subsequent confirmations.
Optimal detection of Salmonella spp.
The IRIS Salmonella media is very easy to use and provides an excellent contrast between agar and pink-magenta Salmonella colonies and the secondary flora, which appear blue to dark blue. Studies performed on IRIS Salmonella® Agar show a high specificity for the detection of Salmonellae including atypical species and serovars which are a source of confusion on other medium. The detection of typhoidal Salmonella, lactose-positive Salmonella and sucrose-positive strains is guaranteed. The agar allows the detection of non-motile serovars and monophasic strains as well as strains that show little to no esterase activity on other media.
How to confirm presumptive Salmonella?
- Conventional tests described in standard methods CEN or ISO (including the purification step) using a colony isolated on IRIS Salmonella® Agar.
- Latex test (CONFIRM Salmonella) made directly from a colony isolated on IRIS Salmonella® Agar
Figure 2. CONFIRM Salmonella latex test kits available from Biokar for the confirmation of presumptive Salmonella spp.
You can download the IRIS Salmonella Technical Datasheet for more information
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