In the world of molecular biology, sanger sequencing has often been considered the gold standard since the 1980s for accurate gene sequencing and is commonly used for the detection of single nucleotide variants to small insertion/deletion mutations. Despite the rise of next-generation sequencing technologies, Sanger sequencing still holds a critical place due to its accuracy, sensitivity, and ability to sequence small fragments with high reliability. The process involves adding fluorescently labelled dideoxynucleotides (ddNTPs), which terminate the DNA chain at specific points, followed by capillary electrophoresis, allowing the identification of each base—adenine (A), cytosine (C), guanine (G), and thymine (T). These terminated fragments are then separated by size through capillary electrophoresis, generating a chromatogram that DNA sequence. When it comes to DNA sequencing, accuracy and consistency are paramount. Whether you’re sequencing genes or verifying mutations, the quality of your sequencing reagent directly impacts the reliability of your results. That’s where Nimagens Brilliant dye® Terminator Dye comes in.
Why choose Nimagens Brilliant dye ® Terminator Dye?
Superior Accuracy
Brilliant dye® kits are drop in replacement terminator dyes that offer unmatched accuracy in sequencing reactions. Known for its precision, this dye ensures that every base is incorporated correctly, providing high-quality reads and reducing sequencing errors providing the reliability you need to produce high-confidence results, making it a great choice for researchers and medical scientists worldwide.
Compatibility with Multiple genetic analysers
One of the key features of Brilliant dye® Terminator Dye is its wide compatibility with a variety of sequencing platforms. It’s a drop in replacement and compatible with a range of genetic analysers including the Applied Biosystems 3130, 3500 and 3730 series, ensuring you can seamlessly integrate it into your existing workflow. This versatility makes it the perfect choice for both experienced labs and those optimising their sanger sequencing workflow, giving you flexibility and reduced cost without sacrificing performance.
Exceptional Sensitivity
BrilliantDye® Terminator Dye is engineered for high sensitivity, making it ideal for low-concentration samples. Whether you’re working with difficult-to-sequence templates, low DNA yield, or rare mutations, the Brilliant dye® system ensures that your sequencing results remain clear. The dye’s sensitivity helps to capture even faint signals, improving the quality of your sequencing reactions and enabling accurate detection of small sequence variations.
Fast, Reliable Results with Minimal Optimization
Brilliant dye® Terminator Dye is designed to provide fast, reproducible sequencing results with minimal optimization. Its reliable chemistry and ease of use reduce the need for extensive troubleshooting or time-consuming protocol adjustments.
High Signal-to-Noise Ratio
One of the standout features of BrilliantDye® Terminator kits is its ability to provide a high signal-to-noise ratio, ensuring clear and easy-to-read sequencing traces. This helps eliminate background noise and minimizes the need for post-sequencing cleanup, saving you time and effort.
If your laboratory is interested in looking at an alternative, cost-effective Terminator dye kit, then contact us today.
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