qPCRBIO SyGreen Mix

qPCRBIO SyGreen Mix, PCR Biosystems, SyGreen Mix, qPCRBIO | Medical Supply Company

qPCRBIO SyGreen Mix from PCR BioSystems combines a proprietary non-inhibiting intercalating dye with the latest advances in polymerase technology and buffer chemistry to give you fast, highly sensitive and reproducible real-time PCR.

qPCRBIO SyGreen Mix can be used to reliably quantify any DNA template including genomic, cDNA and viral sequences, and is able to detect extremely low copy number targets with the highest efficiency.

qPCRBIO SyGreen Mix is designed for fast, highly sensitive and reproducible real-time PCR, with minimal or no optimisation required. The antibody-mediated hot start system prevents the formation of primer dimers and non-specific products leading to greatly improved reaction sensitivity and specificity.

The 2x mix uses a proprietary intercalating dye that does not inhibit PCR, unlike other popular fluorescent dyes. The same developments that improve the sensitivity and consistency of qPCRBIO SyGreen Mix in standard cycling conditions also allow for industry-leading performance in fast and ultra fast cycling conditions.

qPCRBIO SyGreen Mix Features

  • Rapid extension rate for early Ct values
  • Market-leading sensitivity – increased limit of detection
  • Specific amplification from complex templates including GC-rich sequences
  • Non-PCR inhibiting intercalating dye
  • Also available as an easy-to-see blue mix
  • Compatible with all standard and fast cycling real-time instruments

The 2x mix uses a proprietary intercalating dye that does not inhibit PCR, unlike other popular fluorescent dyes. The same developments that improve the sensitivity and consistency of qPCRBIO SyGreen Mix in standard cycling conditions also allow for industry-leading performance in fast and ultra-fast cycling conditions.

qPCRBIO SyGreen Mix Applications

  • Absolute quantification
  • Relative gene expression analysis
  • High-throughput qPCR from genomic, cDNA and viral sequences
  • Detection of extremely low copy number targets
  • Crude sample PCR

Storage

The kit should be stored between -30°C and -15°C. Avoid prolonged exposure to light. If stored correctly the kit will retain full activity for 12 months. The kit can be stored at 4°C for 1 month. The kit can go through 30 freeze/thaw cycles with no loss of activity.

To find out more, get in touch with a product specialist below or browse our range of PCR Biosystems products here